Uncover true single-cell spatial mapping
A new generation of high-precision tools for the life sciences industry. Our technological innovations are the most advanced spatial omics solutions for discovery research and designed for broad accessibility. Plugging directly into existing sequencing workflows, Curio’s spatial mapping technologies are easy to implement and do not require investment in new capital equipment.
Curio Seeker
Spatial Transcriptome Mapping Kit
Based on Slide-seq technology, the Curio Seeker provides a dense, continuous spatial map of RNA expression from any eukaryotic organism for unbiased high-resolution, whole transcriptome spatial mapping in fresh frozen tissues.
Curio Seeker captures any polyadenylated nucleic acid molecules, resulting in an unbiased, whole transcriptome spatial gene expression map. No pre-selection of gene targets nor generation of gene panels are required.
Curio Seeker provides a dense, continuous spatial map of RNA expression from any eukaryotic organism.
Curio Trekker
Single-Cell Spatial Mapping Kit
Based on Slide-tag technology, the Curio Trekker Single-Cell Spatial Mapping Kit delivers true, single-cell spatial mapping by arranging standard, single-cell genomics data into spatial orientation.
Curio Trekker works by tagging each nucleus within its native tissue environment with unique spatial barcodes. These spatial barcodes are read using next-generation sequencing (NGS), allowing each nucleus to be bioinformatically positioned in its spatial coordinates. The result is a spatial map with true single-cell resolution without the use of complex instrumentation, cell-type deconvolution, or cell segmentation.
Curio Trekker Workflow
The workflow begins by melting a frozen tissue section on the Cruio Trekker tile, a spatially barcoded surface. Upon exposure to UV light, oligonucleotides carrying spatial barcodes are cleaved from the beads and attach to the nuclei in their vicinity. Spatially tagged nuclei are fed into standard single-nucleus RNA sequencing (snRNA-seq) workflows. Spatial barcode oligos are captured and amplified alongside cellular RNA. A custom bioinformatics pipeline is used to map the position of each nucleus based on the spatial barcodes it contains.
Curio Seeker Workflow
Once your tissue section is placed on the Curio Seeker tile, mRNA is captured and hybridized on the spatially-indexed beads before reverse transcription. The indexed beads are then dissociated from the tile and the tissue digested, followed by second strand synthesis and cDNA amplification. Purified cDNA undergoes NGS library preparation and is sequenced. The resulting FASTQ files are processed by the Curio Seeker bioinformatics pipeline to create a continuous whole transcriptome spatial map of your tissue section.